- Measurement of CTLL-2 cell proliferation
- Determination of the biological activity of human and murine IL-2 and IL-4 as well as murine IL-12 and IL-18. o Effect of cytotoxic/cytostatic substances (medicinal products, other pharmaceutical agents).
- Checkpoint inhibitor effects
- Screening and specific identification of cell growth inhibitors, such as antibodies, physiological messengers and virus and tumour-induced immune inhibition
WST-1 Cell Proliferation Kit (ready-to-use)
Immunservice’s WST-1 Cell Proliferation Kit allows easy, fast and accurate measurement of cell proliferation. Quantification is based on cleavage of the water-soluble tetrazolium salt WST-1 to a formazan dye by viable cells. An increase in the cell count leads to formation of a larger amount of the dye, which is quantified by measurement of the absorbance at 420 – 480 nm. The absorbance level correlates strongly with the number of viable cells in the assay.
In combination with our detailed directions for use you will find here the solution for the success of your cell proliferation and cell inhibitions experiments.
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For research use only
Please note also our Ordering and Shipping Information.
Storage at -20°C. To avoid repeated freezing and thawing, the solution should be divided into aliquots (1 mL is sufficient for one 96-well microtiter plate). When properly stored, the stability of the WST-1 Kit is guaranteed for 6 months.
Berridge, M. V., Herst, P. M. & Tan, A. S. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Annu. Rev. 11, 127–152 (2005)
Further references on request at Research@immunservice.com
Below you can download as a PDF document all relevant documents on the respective product.
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Service: IL-2 Bioactivity measurements
On demand
8-10 weeks after sample receipt. Express measurements are available at an extra cost.