| Unit | 1 x 10 ml, 1 x 25 ml, 4 x 25 ml |
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WST-1 CTLL-2 Proliferation Kit (ready-to-use)
Immunservice’s WST-1 CTLL-2 Proliferation Kit has been optimized for use with CTLL-2 cells. CTLL-2 cells are a cytotoxic T cell line that is very suitable and often used for highly sensitive measurement of the biological activity of the cytokine interleukin-2 (IL-2) as well as the cytokines IL-4, IL-12 and IL-18. Quantification is via determination of the proliferation strength as the response to the cytokine concentration. The WST-1 CTLL-2 Proliferation Kit allows easy, fast and accurate measurement of this cell proliferation. Quantification is based on cleavage of the water-soluble tetrazolium salt WST-1 to a formazan dye by viable cells. A proliferative increase in the cell count leads to formation of a larger amount of the dye, which is quantified by measurement of the absorbance at 420 – 480 nm. The absorbance level correlates strongly with the number of viable cells in the assay.
In combination with our detailed directions for use you will find here the solution for the success of your cell proliferation and cell inhibition experiments with CTLL-2 cells.
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For research use only
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Additional Information
Areas of Application
- Measurement of CTLL-2 cell proliferation
- Determination of the biological activity of human and murine IL-2 and IL-4 as well as murine IL-12 and IL-18. o Effect of cytotoxic/cytostatic substances (medicinal products, other pharmaceutical agents).
- Checkpoint inhibitor effects
- Screening and specific identification of cell growth inhibitors, such as antibodies, physiological messengers and virus and tumour-induced immune inhibition
Storage and Stability
Storage at -20°C. To avoid repeated freezing and thawing, the solution should be divided into aliquots (1 mL is sufficient for one 96-well microtiter plate). When properly stored the stability of the WST-1 Kit is guaranteed for 6 months.
Storage at -20°C. To avoid repeated freezing and thawing, the solution should be divided into aliquots (1 mL is sufficient for one 96-well microtiter plate). When properly stored the stability of the WST-1 Kit is guaranteed for 6 months.
References
1. Berridge, M. V., Herst, P. M. & Tan, A. S. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Annu. Rev. 11, 127–152 (2005)
2. Davis, L. S., Lipsky, P. E. & Bottomly, K. Measurement of human and murine interleukin 2 and interleukin 4. Curr. Protoc. Immunol. Chapter 6, Unit 6.3 (2001)
3. Khatri A1, Husaini Y, R. P. Murine CTLL-2 cells respond to mIL12: prospects for developing an alternative bioassay for measurement of murine cytokines IL12 and IL18. J. Immunol. Methods 326, 41–53 (2007)
Further references on request at Research@immunservice.com
1. Berridge, M. V., Herst, P. M. & Tan, A. S. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Annu. Rev. 11, 127–152 (2005)
2. Davis, L. S., Lipsky, P. E. & Bottomly, K. Measurement of human and murine interleukin 2 and interleukin 4. Curr. Protoc. Immunol. Chapter 6, Unit 6.3 (2001)
3. Khatri A1, Husaini Y, R. P. Murine CTLL-2 cells respond to mIL12: prospects for developing an alternative bioassay for measurement of murine cytokines IL12 and IL18. J. Immunol. Methods 326, 41–53 (2007)
Further references on request at Research@immunservice.com
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