| Unit | IL2.160606.1 (1 x 10^4 IU/ml), IL2.160606.2 (1 x 10^5 IU/ml) |
|---|
Interleukin-2, human (Aldesleukin)
Recombinant, expressed in Escherichia coli (E.coli)
This recombinant human interleukin-2 (rhIL-2, Aldesleukin) is an immunomodulatory cytokine with a molecular weight of approximately 15 kDa. Aldesleukin differs from the natural Interleukin-2: Aldesleukin does not have an N-terminal alanine, the amino acid cysteine at position 125 is substituted by serine and the molecule is not glycosylated. The biological activity of the recombinant IL-2 is similar to that of the native human IL- 2. Interleukin-2 is important for proliferation of T and B lymphocytes and stimulates differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.
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Additional Information
Areas of Application
Recombinant human IL-2 can be used for the culture of IL-2- dependent cells and for activation of immune cells.
Possible applications are:
Recombinant human IL-2 can be used for the culture of IL-2- dependent cells and for activation of immune cells.
Possible applications are:
- Culture of human and murine IL-2-dependent primary T cells, T cell lines and NK cells
- Proliferation of mitogen-activated T lymphocytes and NK cells
- Establishment of human and murine thymocyte, splenocyte or peripheral blood lymphocyte derived T-cell lines
- Generation of human and murine lymphokine-activated killer (LAK) cells
Storage
rhIL-2 should be stored at -20°C or -80°C. Avoid repeated freeze-thaw cycles. If stored accordingly, this recombinant human Interleukin-2 is stable for at least 6 months.
rhIL-2 should be stored at -20°C or -80°C. Avoid repeated freeze-thaw cycles. If stored accordingly, this recombinant human Interleukin-2 is stable for at least 6 months.
Ingredients
Bioactive recombinant human interleukin-2 in RPMI 1640 medium supplemented with human serum albumin (HSA). If necessary, dilution is recommended in media supplemented with HSA, BSA or FCS.
Bioactivity
The specific activity corresponds to a value > 6 x 106 IU/mg. The activity was determined by a dose dependent proliferation of the murine cytotoxic T-cell line CTLL-2 and is expressed relative to the 2nd WHO International Standard for Interleukin- 2.
Bioactive recombinant human interleukin-2 in RPMI 1640 medium supplemented with human serum albumin (HSA). If necessary, dilution is recommended in media supplemented with HSA, BSA or FCS.
Bioactivity
The specific activity corresponds to a value > 6 x 106 IU/mg. The activity was determined by a dose dependent proliferation of the murine cytotoxic T-cell line CTLL-2 and is expressed relative to the 2nd WHO International Standard for Interleukin- 2.
References
1. Olejniczak K, Kasprzak. A. Biological properties of interleukin 2 and its role in pathogenesis of selected diseases - a review. Med. Sci. Monit. 2008;14(10):RA179–A189.. J. Immunol. Methods 65, 55–63 (1983).
2. Boyman O, Sprint J. The role of interleukin-2 during homeostasis and activation of the immune system.. Nat. Rev. Immunol. 2012;12(3):180–190. 3. Fenwick BW1, Schore CE, O. B. Human recombinant interleukin-2(125) induced in vitro proliferation of equine, caprine, ovine, canine and feline peripheral blood lymphocytes. Comp Immunol Microbiol Infect Dis. 11, 51– 60 (1988).
Other references on request at Research@immunservice.com
1. Olejniczak K, Kasprzak. A. Biological properties of interleukin 2 and its role in pathogenesis of selected diseases - a review. Med. Sci. Monit. 2008;14(10):RA179–A189.. J. Immunol. Methods 65, 55–63 (1983).
2. Boyman O, Sprint J. The role of interleukin-2 during homeostasis and activation of the immune system.. Nat. Rev. Immunol. 2012;12(3):180–190. 3. Fenwick BW1, Schore CE, O. B. Human recombinant interleukin-2(125) induced in vitro proliferation of equine, caprine, ovine, canine and feline peripheral blood lymphocytes. Comp Immunol Microbiol Infect Dis. 11, 51– 60 (1988).
Other references on request at Research@immunservice.com
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